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相关概念视频

G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

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相关实验视频

Updated: May 7, 2026

Pull-down of Calmodulin-binding Proteins
07:51

Pull-down of Calmodulin-binding Proteins

Published on: January 23, 2012

NCAM和N-cadherin的形态调节活动可以通过依赖G蛋白激活L型和N型神经元Ca2+通道来解释.

P Doherty1, S V Ashton, S E Moore

  • 1Department of Experimental Pathology, UMDS, Guy's Hospital, London, England.

Cell
|October 4, 1991
PubMed
概括
此摘要是机器生成的。

神经细胞粘附分子 (NCAM) 和N-cadherin通过激活细胞内通路直接触发细胞形状变化并改变细胞表型. 这些细胞粘附分子中介于PC12细胞中快速的,转录独立的神经元分化.

更多相关视频

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
11:02

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Published on: August 13, 2021

相关实验视频

Last Updated: May 7, 2026

Pull-down of Calmodulin-binding Proteins
07:51

Pull-down of Calmodulin-binding Proteins

Published on: January 23, 2012

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
11:02

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Published on: August 13, 2021

科学领域:

  • 细胞生物学 细胞生物学
  • 神经科学是一个神经科学.
  • 分子生物学分子生物学

背景情况:

  • 细胞粘附分子 (CAMs) 在调节细胞行为方面发挥着关键作用.
  • 神经细胞粘附分子 (NCAM) 和N-cadherin是参与神经发育的关键CAM.
  • 在CAM中介形态调节的基础上,细胞内信号传递机制尚未完全理解.

研究的目的:

  • 研究NCAM和N-cadherin在调节细胞形态和表型中的作用.
  • 阐明涉及CAM介导信号传递的细胞内第二信使通路.
  • 提供CAMs通过外膜信号传递的直接证据.

主要方法:

  • 在表达N-cadherin或NCAM的3T3细胞单层上培养PC12细胞.
  • 评估了形态变化,并测量了特定蛋白质免疫活性 (Thy-1,L1/NILE,低亲和性NGF受体).
  • 评估了百日咳毒素,通道抗体和激酶抑制剂 (K-252b) 的作用.

主要成果:

  • NCAM和N-cadherin诱导了PC12细胞从上腺转移到神经元表型的快速转录独立转移.
  • 这些CAMs特别增加了Thy-1免疫反活性.
  • 形态反应被百日咳毒素和通道阻断剂以及K-252b抑制,这表明G蛋白和信号参与,但不是广谱激酶.

结论:

  • 像NCAM和N-cadherin这样的细胞粘附分子可以直接改变细胞表型.
  • 涉及G蛋白和的跨膜信号通路调解了NCAM和N-cadherin诱导的形态和生化反应.
  • 这些发现突出了细胞相互作用的新机制,用于调节细胞命运和分化.