Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

65.9K
This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal...
65.9K
Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure08:40

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

8.0K
A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is...
8.0K
Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function11:47

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

11.1K
We present a biochemical and behavioral protocol to evaluate the efficacy of mitochondria-targeted water-soluble compounds for the treatment of Spinocerebellar ataxia type 1 (SCA1) and other cerebellar neurodegenerative diseases.
11.1K
Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain05:51

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

20.3K
Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.
20.3K
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

13.9K
Ascorbate plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. Here we describe a medium-throughput, specific and inexpensive microplate assay for the determination of both intra- and extracellular ascorbate in cell culture.
13.9K
Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

61.6K
We describe a method to target drugs to the central nervous system by either implanting a catheter or performing a bolus injection into the right lateral ventricle in mice. We focus specifically on the delivery of antisense oligonucleotides. This technique is readily adaptable to other drugs and to...
61.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Clinical Impact of Intravascular Imaging-Guided Percutaneous Coronary Intervention in Acute Myocardial Infarction Patients with High Thrombus Burden.

The American journal of cardiology·2025
Same author

Comparison of High-Dose versus Low-Dose Paclitaxel Drug-Coated Balloons for Native Femoropopliteal Artery Disease: An Analysis of the K-VIS ELLA Registry.

Yonsei medical journal·2025
Same author

Disparities in Heart Transplantation Allocation and Outcomes by Blood Type in Korea (2010-2022).

Korean circulation journal·2025
Same author

Interpreting clinical outcomes using different strut thickness in coronary artery disease: insights from vascular imaging analysis.

Frontiers in cardiovascular medicine·2025
Same author

Electrocardiographic-Driven artificial intelligence Model: A new approach to predicting One-Year mortality in heart failure with reduced ejection fraction patients.

International journal of medical informatics·2025
Same author

Potent P2Y<sub>12</sub> inhibitors in patients with acute myocardial infarction and cardiogenic shock.

Critical care (London, England)·2025

相关实验视频

Updated: Jan 9, 2026

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

65.9K

一种对缺氧反应的分子,因特林结合激酶,通过招募内皮前体细胞到缺血组织的前体细胞来控制产后血管生成.

Seung-Pyo Lee1, Seock-Won Youn, Hyun-Jai Cho

  • 1Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongro-gu, Seoul 110-744, South Korea.

Circulation
|July 5, 2006
PubMed
概括

整体连结激酶 (ILK) 是内皮前体细胞 (EPC) 招募到缺血组织的新型调节剂. 在低氧状态下,ILK控制着粘附分子的表达,促进新血管化.

更多相关视频

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 12, 2014

13.9K
Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Published on: May 12, 2013

61.6K

相关实验视频

Last Updated: Jan 9, 2026

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

65.9K
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 12, 2014

13.9K
Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Published on: May 12, 2013

61.6K

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生理学 生理学 生理学

背景情况:

  • 内皮原生细胞 (EPC) 招募到缺氧内皮细胞 (ECs) 对于缺血组织的血管生成至关重要.
  • 在低氧和随后的EPC招募下,调节EC中粘附分子表达的信号通路在很大程度上是未知的.
  • 在这个过程中,综合素连接激酶 (ILK) 被确定为一种新型的介质.

研究的目的:

  • 在低毒条件下研究内源性整合素结合激酶 (ILK) 在内皮细胞 (EC) 中的作用.
  • 阐明涉及ILK在粘附分子调节和EPC招募中的信号通路.
  • 评估ILK调节在缺血组织新血管化中的治疗潜力.

主要方法:

  • 在低氧条件下的EC中ILK表达和活性的时间依赖性分析.
  • 通过热冲击蛋白调节ILK的研究 90.
  • 评估ILK在通过核因子-kappaB (NF-κB) 和缺氧诱导因子-1alpha (HIF-1α) 调节树皮细胞衍生因子-1 (SDF-1) 和细胞间粘附分子-1 (ICAM-1) 的表达中的作用.
  • 使用后肢缺血模型和Matrigel插头进行体内研究,以评估ILK阻断或过度表达对EPC定位和新血管化的影响.

主要成果:

  • 缺氧在EC中依赖时间上调了内源ILK表达和激酶活性,增加了ICAM-1和SDF-1水平.
  • 通过热冲击蛋白90的稳定来调节ILK的上调.
  • 在正常的EC中ILK过度表达通过NF-κB和HIF-1α上调ICAM-1和SDF-1.
  • 低氧EC中的ILK阻断显著降低了ICAM-1和SDF-1的表达,降低了EPC的整合.
  • 在体内,ILK阻断损害了缺血性四肢的EPC指导和新血管化,而ILK过度表达改善了它.

结论:

  • 内源性ILK作为一种新的上游反应者,对ECs的低氧压力做出反应.
  • ILK在控制参与EPC招募到缺血组织的关键分子的表达方面发挥着关键作用.
  • 向ILK是一个潜在的治疗策略,用于增强缺血性疾病中的新血管化.