转化生长因子-β1调节组织工程支架中的内皮细胞原生细胞的细胞外基质生产,增殖和亡
Virna L Sales1, George C Engelmayr, Bret A Mettler
1Department of Cardiac Surgery, Children's Hospital Boston, 300 Longwood Ave, Boston, Massachusetts 02115, USA.
Circulation
|July 6, 2006
概括
转化生长因子-β1 (TGF-β1) 促进内皮原生细胞 (EPC) 在组织工程支架中形成细胞外基质. 这种细胞转化增强了组织形成,用于潜在的心脏门修复.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 组织工程是组织工程.
背景情况:
- 内皮细胞和循环内皮原生细胞 (EPCs) 可以表现出从内皮细胞到介质细胞状态的表型转变.
- 研究了EPCs在组织工程支架内增强细胞外矩阵形成的能力,当它们被转化生长因子-β1 (TGF-β1) 刺激时.
研究的目的:
- 确定TGF-β1是否可以诱导在组织工程支架上播种的EPCs的表型变化.
- 评估TGF-β1诱导的EPCs对细胞外基质生产和支架内的组织形成的影响.
主要方法:
- 绵羊外周血液EPCs是在多糖酸/多4-基酸) 支架上培养的.
- 支架在不同的细胞密度和化时间下与TGF-β1或没有TGF-β1进行化.
- 免疫光学,西式涂抹,扫描电子显微镜和组织学被用于分析.
主要成果:
- 在对TGF-β1.1的反应中,EPCs表现出从内皮细胞转变为介质细胞标记物的表型转变.
- 刺激TGF-β1导致组织形成的增强,DNA含量增加,拉米宁和纤维菌素的表达上调.
- 在经过TGF-β1治疗的支架中,观察到 I 和 III 类型的原蛋白显著增加,以及托波拉斯的合成.
结论:
- 用TGF-β1进行EPC种子支架的体外刺激,促进有组织的细胞架构和矩阵合成 (葡萄糖蛋白,原蛋白,弹性质).
- 这种方法结合了孤立的EPC和TGF-β1,为指导工程心脏膜组织形成提供了一个有希望的策略.
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