通过Drosophila胚胎中的重叠激活剂和抑制剂来调节细分条纹
D Stanojevic1, S Small, M Levine
1Department of Biological Sciences, Fairchild Center, Columbia University, New York, NY 10027.
Science (New York, N.Y.)
|November 29, 1991
概括
虫胚胎中的基因表达是由特定的DNA序列控制的. 这些序列就像基因开关一样,使用激活器和抑制器精确地打开和关闭基因表达.
科学领域:
- 发育生物学是发展生物学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 在Drosophila melanogaster胚胎中的基因表达模式可以作为理解真核生物基因调节的模型.
- 已知偶跳过 (eve) 条纹2表达受到几个关键发育基因的影响.
研究的目的:
- 为了调查平跳过 (eve) 条纹2促进体和其表达模式中的调节性蛋白质结合位点之间的直接联系.
- 阐明特定的转录因子在激活和抑制eve stripe 2表达中的作用.
主要方法:
- 对Drosophila melanogaster胚胎基因组的分析.
- 在eve条纹2促销元件内对转录调节器的结合位点的识别和表征.
- 结合部位占用与观察到的基因表达模式的相关性.
主要成果:
- 双体 (bcd),凸体 (hb),克鲁佩尔 (Kr) 和巨型 (gt) 蛋白质的结合部位与条纹2表达模式之间建立了直接相关性.
- 发现BCD和HB蛋白质的结合部位能够调节基因激活.
- 对GT和Kr蛋白质的邻近结合点被确定为抑制剂,对于建立条纹边界至关重要.
结论:
- 均跳过的 (eve) 条纹2促销元件作为遗传上的开关起作用.
- 激活剂 (bcd, hb) 和抑制剂 (gt, Kr) 的组合作用精确地控制胚胎发生过程中的基因表达边界.
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