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相关概念视频

The Nucleosome Core Particle02:10

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...

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相关实验视频

Updated: Jul 6, 2026

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

需要博士学位才能读取组素代码.

Jane Mellor1

  • 1Department of Biochemistry, South Parks Road, Oxford, OX1 3QU, UK. jane.mellor@bioch.ox.ac.uk

Cell
|July 15, 2006
PubMed
概括
此摘要是机器生成的。

植物宿主群指蛋白解释基因编码,这是调节基因活动的修改模式. 这些蛋白与像H3K4三甲基化这样的基因基因修饰相互作用,以影响基因表达和抑制.

更多相关视频

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
09:43

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

相关实验视频

Last Updated: Jul 6, 2026

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
09:43

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 遗传学 是一个遗传学.

背景情况:

  • 基因组修饰形成了"基因组代码",调节染色质状态和基因转录.
  • 植物主体 (PHD) 指蛋白是这个表观遗传代码的关键读者.
  • 在lysine 4 (H3K4me3) 中三甲基化基因3是活性基因促进者的标志.

研究的目的:

  • 探索植物本地主体 (PHD) 指蛋白如何解释特定的基因素修饰.
  • 阐明PHD手指在读取基因组代码中的作用及其对基因调节的影响.
  • 了解PHD手指与三甲基化素4在3基因素 (H3K4me3) 上的相互作用.

主要方法:

  • 关于PHD指蛋白和基因组修饰的最新文献的综述.
  • 分析蛋白质-海斯相互作用机制.
  • 研究PHD指部结合H3K4me3.3的功能后果.

主要成果:

  • PHD手指蛋白识别并结合到特定的组织蛋白标记,包括H3K4me3.3.
  • 这种相互作用可以促进基因表达.
  • 有证据表明,PHD手指也可能参与基因抑制.
  • H3K4me3是PHD手指在活跃基因位点读取的关键信号.

结论:

  • PHD指蛋白在解码基因组码中起着至关重要的作用.
  • 通过PHD手指与H3K4me3的相互作用有助于调节转录活动.
  • PHD手指在基因表达和抑制中的双重作用凸显了它们在表观遗传学中的复杂功能.