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相关概念视频

Protein Organization01:13

Protein Organization

Overview
Protein Organization01:13

Protein Organization

Overview
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.

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相关实验视频

Updated: Jul 6, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

从一个变质蛋白质的多重对齐张量.

Erika B Gebel1, Ke Ruan, Joel R Tolman

  • 1Department of Biological Chemistry, Johns Hopkins University, School of Medicine,1915 East Madison, Baltimore, Maryland 21205, USA.

Journal of the American Chemical Society
|July 20, 2006
PubMed
概括

研究人员使用残余二极合 (RDC) 研究了变质核酶的残余结构. 分析显示至少有三个独立的对齐张量,表明在变质状态下有一个不对称的整体平均结构.

科学领域:

  • 生物物理学的生物物理.
  • 结构生物学 结构生物学
  • 蛋白质动力学 蛋白质动力学

背景情况:

  • 变质蛋白质状态的结构性表征仍然不完整.
  • 剩余二极合 (RDCs) 在特定的对齐条件下提供了对蛋白质结构的洞察.

研究的目的:

  • 为了进一步阐明非天然核酶的残余结构.
  • 使用RDC测量量量化核酶中的独立对齐张量数.

主要方法:

  • 在八个数据集中从4M尿素中的核酶中收集脊柱胺15N-1H RDC.
  • 使用奇数值分解 (SVD) 分析RDC数据以确定对齐张量维度.

主要成果:

  • SVD分析表明至少存在三个独立的对齐张量.
  • 正角线性组合 (OLC) -RDC数据集证实了这三种张量器的稳定性.
  • RDC数据跨越了三个或更多的维度,表明整体平均结构中的不对称性.

结论:

  • 核酶的变质状态表现出一个整体平均结构,至少有三个不对称的维度.
  • 这些发现与先前在变质核酶中具有原生类型拓学的观察结果一致.

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The MultiBac Protein Complex Production Platform at the EMBL
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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

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