限制中心体重复的机制是每个细胞周期只能重复一次
Meng-Fu Bryan Tsou1, Tim Stearns
1Department of Biological Sciences, Stanford University, Stanford University Medical School, Stanford, California 94305-5020, USA.
Nature
|July 25, 2006
概括
中心细胞复制是由中心脱离控制的,这需要在期内分离蛋白酶. 这一过程允许在随后的细胞周期中产生新的中心生长,防止重复复制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 中心体由中心体和中心体材料组成,组织微管细胞骨架.
- 细胞循环的进展涉及单一一轮的中心体重复,确保一个中心体每细胞在线粒分裂之前.
- 新形成的中心离子一直与现有的中心离子保持联系,直到线粒分裂晚期或G1早期,但这种脱离的机制和时间尚不清楚.
研究的目的:
- 阐明控制中心点参与和脱离的机制.
- 了解中心点接触/脱离和中心点重复的可能性之间的关系.
- 为了确定参与许可中心极复制的分子参与者.
主要方法:
- 使用Xenopus蛋提取物和纯化的中心的体外系统的开发.
- 分析中心点脱离和增长动态的分析.
- 研究分离酶,亚纳酶促进复合体和Cdk2/cyclin E.的作用.
主要成果:
- 在酶中,基离合的脱取决于酶促进复合体和蛋白酶分离酶.
- 新的中心球生长发生在介相期间,需要先前脱离的中心球和Cdk2/cyclin E活动.
- 中心参与防止在同一个细胞周期内重复复制.
结论:
- 通过分离在亚纳相中脱离中心分离,在下一个细胞周期中许可中心重复.
- 中心细胞复制的细胞周期控制依赖于暂时将许可 (亚纳相) 与生长 (S相) 分开.
- 隔离酶在中心点脱和姐妹染色体分离中的作用可以防止过早脱,并保持基因组的稳定性.
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