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相关概念视频

Mismatch Repair01:36

Mismatch Repair

Overview
Mismatch Repair01:36

Mismatch Repair

Overview
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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FBH1 and RAD54L directly interact and cooperate to drive replication fork reversal.

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相关实验视频

Updated: Jun 27, 2026

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

Rad54蛋白质促进了霍莱德结的分支迁移.

Dmitry V Bugreev1, Olga M Mazina, Alexander V Mazin

  • 1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102-1192, USA.

Nature
|July 25, 2006
PubMed
概括

研究人员确定Rad54蛋白质是同源重组的一个关键参与者. 这种蛋白质特别结合和迁移霍莱德结,这对于DNA修复和真核细胞的染色体分离至关重要.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 同源重组对于DNA双链断裂修复和染色体分离至关重要.
  • 霍莱德结 (HJs) 是同源重组的关键中间体,但未知的是真核细胞分支迁移蛋白.
  • 以前的研究表明,Rad54在同源重组的后期阶段起作用.

研究的目的:

  • 为了识别难以捉摸的真核蛋白质,负责霍莱德交叉分支迁移.
  • 描述Rad54与霍莱德连接有关的生物化学活性.

主要方法:

  • 生物化学试验测试Rad54与HJ类结构的结合.
  • ATPase测试测量Rad54的分支迁移活动.
  • 人类和酵母Rad54正方体的比较.

主要成果:

  • Rad54蛋白特别结合于类似于霍莱德结的结构.
  • Rad54以ATPase依赖的方式促进HJs的双向分支迁移.
  • 这种活性在人体和酵母Rad54中保持不变,与同源重组的晚期作用一致.

结论:

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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence

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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

相关实验视频

Last Updated: Jun 27, 2026

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

  • Rad54被确定为在真核生物中同源的重组分支迁移蛋白.
  • 这一发现澄清了DNA修复机制中长期存在的.
  • 保存的分支迁移活动支持Rad54在同源重组的后期阶段的作用.