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相关概念视频

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

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相关实验视频

Updated: Jul 9, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

通过与NFATAT的合作,FOXP3控制了调节性T细胞功能.

Yongqing Wu1, Madhuri Borde, Vigo Heissmeyer

  • 1Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA.

Cell
|July 29, 2006
PubMed
概括

调节性T细胞 (Tregs) 使用独特的NFAT-FOXP3复合体来抑制免疫反应. 这种相互作用开关T细胞激活程序,使Treg抑制功能和治疗自身免疫糖尿病.

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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

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In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation
10:21

In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation

Published on: June 9, 2026

相关实验视频

Last Updated: Jul 9, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
08:02

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Published on: January 22, 2020

In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation
10:21

In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation

Published on: June 9, 2026

科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 结构生物学 结构生物学

背景情况:

  • 激活T细胞的核因子 (NFAT) 对于T细胞激活至关重要.
  • NFAT通常与AP-1转录因子合作,调节T细胞基因.
  • 调控性T细胞 (Tregs) 具有不同的功能,需要独特的分子机制.

研究的目的:

  • 为了研究底层调节性T细胞 (Treg) 功能的分子机制.
  • 确定在Tregs.中与NFAT合作的转录因子.
  • 阐明NFAT-FOXP3相互作用的结构基础及其功能后果.

主要方法:

  • 用X射线晶体学来确定NFAT:FOXP2:DNA复合物的结构.
  • 基于结构见解的FOXP3的局部定向突变发生.
  • 在小鼠模型中测量基因表达 (IL2,CTLA4,CD25) 和抑制剂活性的功能性测试.

主要成果:

  • 在Tregs.中确定了NFAT和FOXP3之间的新型合作综合体.
  • 揭示了NFAT和FOXP3 (使用FOXP2结构) 之间广泛的蛋白质-蛋白质相互作用接口.
  • 证明破坏NFAT-FOXP3相互作用会损害Treg功能,包括IL2抑制和抑制活性.

结论:

  • 在Tregs中,NFAT与FOXP3合作,与其在传统T细胞中与AP-1的作用不同.
  • 这种NFAT-FOXP3复合体对于Treg介导的免疫抑制至关重要.
  • 准NFAT-FOXP3相互作用为自身免疫性疾病提供了潜在的治疗策略.