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相关概念视频

Crossing Over01:34

Crossing Over

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Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
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Olfaction01:25

Olfaction

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The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...
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Exon Recombination02:32

Exon Recombination

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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Crossing Over01:30

Crossing Over

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Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
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Olfactory Receptors: Location and Structure01:03

Olfactory Receptors: Location and Structure

10.4K
The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
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Physiology of Smell and Olfactory Pathway01:20

Physiology of Smell and Olfactory Pathway

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Humans detect odors with the help of specialized cells located in the upper part of the nasal cavity, called olfactory receptor neurons (ORNs). ORNs possess hair-like structures called cilia, which are receptive to sensations from the inhaled air. When an odorant molecule binds to a specific receptor on the cell of the cilia, it leads to a series of events that ultimately cause the ORN to send electrical signals to the olfactory bulb in the brain through the olfactory nerves.
The olfactory...
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相关实验视频

Updated: Apr 28, 2026

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
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High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity

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染色体间相互作用和嗅觉受体选择

Stavros Lomvardas1, Gilad Barnea, David J Pisapia

  • 1Department of Biochemistry and Molecular Biophysics and Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.

Cell
|July 29, 2006
PubMed
概括

14号染色体上的单个增强元件 (H) 与嗅觉受体 (OR) 基因促进体有关. 这种增强剂可以控制感官神经元中仅一个OR基因的随机激活.

科学领域:

  • 嗅觉系统生物学
  • 遗传学和基因组学 遗传学和基因组学
  • 基因调节的分子机制

背景情况:

  • 在个体感官神经元中嗅觉受体 (OR) 基因表达对嗅觉系统的组织和功能至关重要.
  • ORs形成了一个大基因家族,通常每个神经元只表达一个OR基因.
  • 了解控制单个OR基因表达的调节机制是嗅觉研究的关键.

研究的目的:

  • 研究增强剂元素在调节嗅觉受体基因表达中的作用.
  • 为了确定一个特定的增强元件 (H) 与OR基因促进体的相互作用.
  • 阐明控制单个OR基因在嗅觉神经元中的表达选择的机制.

主要方法:

  • 染色体构造捕获 (3C) 用于确定增强元件和OR基因促进体之间的相互作用.
  • 用DNA和RNA光 in situ杂交 (FISH) 来可视化增强剂-促进剂相互作用的物理关联和转录状态.
  • 创建了转基因小鼠模型,以研究额外的增强元件对OR表达的影响.

主要成果:

  • 染色体14上的H增强元件被证明与位于不同染色体上的OR基因促进体具有特定的关联.
  • 鱼实验证实了H增强剂与感觉神经元中单个活跃转录的OR等位基因的局部化.
  • 具有额外H元素的转基因小鼠表现出通常表达OR伪基因的神经元中的第二个功能受体的表达.

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High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
12:02

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Published on: June 2, 2014

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Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
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Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

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Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes
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Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes

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结论:

  • 一个单一的转化作用增强剂元素 (H) 可以调解与多个OR基因促进者的特定关联.
  • 增强剂在嗅觉感官神经元中单个OR等位基因的随机激活中起着关键作用.
  • 这些发现提出了一个模型,其中单个增强剂调节嗅觉受体基因选择.