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Nephrotic Syndrome I : Introduction
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在胚胎小鼠心脏中,心房环的重新进入
Miguel Valderrábano1, Fuhua Chen, Amish S Dave
1Department of Medicine, David Geffen School of Medicine at UCLA, 675 Charles Young Dr So, 3645 MRL, Los Angeles, Calif. 90095-1760, USA.
Circulation
|August 2, 2006
概括
心房 (AV) 环可以在发育中的心脏中自主刺激后支持重新进入心律不整. 这是由于离散导电而发生的,需要临界成熟才能持续重新进入.
科学领域:
- 发育生物学是发展生物学.
- 心脏电生理学心脏电生理学
- 心血管研究的心血管研究.
背景情况:
- 在胚胎发育过程中,在传导系统出现后,心房 (AV) 传导从底部到顶部转移到顶部到底部.
- 尽管AV环不再对AV导电过渡后至关重要,但它可能仍然具有刺激性,并作为心律失常的基质.
- 本研究研究了在这个发育过渡期间的AV导电及其对自主调节的易感性.
研究的目的:
- 研究AV环作为心脏发育期间心律失常的基质的作用.
- 检查自主刺激对胚胎心脏中AV导电和再入的影响.
- 为了确定AV环可以支持持续的再进入的发展阶段.
主要方法:
- 在胚胎小鼠心脏中使用CCD摄像头同时绘制电压和 (Ca2+) 地图 (n=43).
- 在一个较大的队列 (n=309) 中使用fluo-3AM进行孤立映射.
- 用异二醇和卡巴胆醇进行药理刺激,以评估自主调节.
主要成果:
- 电压和Ca2+映射中的传播模式是一致的.
- 在基底条件下,心律失常很少发生,但随着异二醇的使用,心律失常增加.
- 联合上腺和肌肉刺激诱导了解离的前向和逆向AV环传导,导致30个 (8.6%) 心脏在E10.5-E11.5.5时重新进入心脏.
- 重新进入发生在AV环周围,持续数分钟.
- 在早期阶段 (E9.5) 的心脏中没有观察到AV环再进入.
结论:
- 在联合上腺和肌肉刺激过程中,通过空间分离的导电,可以重新进入心房环.
- 显著的心脏成熟,超过胚胎9.5天,是必要的,使AV环维持重新进入心律失常.
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