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H S Phillips¹, J M Hains, G R Laramee

¹Department of Developmental Biology, Genentech, South San Francisco, CA 94080.

Science (New York, N.Y.)

|October 12, 1990

概括

大脑衍生神经营养因子 (BDNF) 和神经营养因子-3 (NT3) 是关键的神经营养因子. BDNF mRNA广泛分布在像海马体和杏仁体这样的老鼠大脑点中,这表明它在这些区域起着至关重要的作用.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

大脑衍生神经营养因子 (BDNF) 和神经营养因子-3 (NT3) 是与神经生长因子相关的同源神经营养因子.
这些神经营养因子在大脑中表现出不同的目标特异性.

研究的目的:

为了研究大脑中BDNF和NT3的信使RNA (mRNA) 的分布模式.
为了确定BDNF和NT3.3的潜在中枢神经系统目标.

主要方法:

在现场杂交被用来检测和定位脑组织中的特定mRNA分子.
用BDNF和NT3的反意义探针可视化它们各自的mRNA表达模式.

主要成果:

在海马体,杏仁体和新皮质中发现了大量的BDNF mRNA,这是大鼠基本前脑胆固醇系统的主要目标.
在嗅觉系统相关结构中也检测到强烈的BDNF mRNA信号.
前脑中的NT3mRNA分布显着更受限制,特别是在海马内 (CA2,中间CA1,牙状).
人类海马体中的BDNF mRNA表达反映了在老鼠海马体中观察到的分布.

结论:

BDNF mRNA的广泛分布表明它在基底前脑胆固醇系统和嗅觉通路中起着重要作用.
BDNF和NT3的独特表达模式突出显示了它们在大脑中的特殊功能.
研究结果表明,基底前脑胆能神经元和嗅觉通路是BDNF的潜在中心目标.

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