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相关概念视频

ATP Synthase: Structure01:18

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
Microtubules in Cell Motility01:24

Microtubules in Cell Motility

Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
The Structure of Intermediate Filaments01:19

The Structure of Intermediate Filaments

The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm).  These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate filaments...
Microtubules in Cell Motility01:24

Microtubules in Cell Motility

Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
Flagella and Motility in Bacteria01:18

Flagella and Motility in Bacteria

Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...
Fimbriae, Pili, and Axial Filaments01:28

Fimbriae, Pili, and Axial Filaments

Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...

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相关实验视频

Updated: Jun 7, 2026

Biophysical Characterization of Flagellar Motor Functions
06:08

Biophysical Characterization of Flagellar Motor Functions

Published on: January 18, 2017

完整的Treponema primitia 鞭毛运动器的 in situ 结构.

Gavin E Murphy1, Jared R Leadbetter, Grant J Jensen

  • 1Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.

Nature
|August 4, 2006
PubMed
概括

研究人员可视化了细菌的鞭毛电机.

科学领域:

  • 微生物学 微生物学
  • 结构生物学 结构生物学
  • 纳米技术纳米技术

背景情况:

  • 细菌的鞭毛电机是一个复杂的纳米机器,使细菌能够运动.
  • 它的结构包括一个定位器和一个旋转器,对定位器的现场组织和相互作用的了解有限.
  • 之前的研究主要集中在转子部件上.

研究的目的:

  • 为了确定完整的细菌鞭毛电机的 in situ 结构.
  • 为了阐明状态器的组装,对称性和细胞内的相互作用.
  • 调查扭矩产生和方向变化的结构基础.

主要方法:

  • 使用电子冷图,对Treponema primitia的整个细胞进行了成像.
  • 进行了20个单个运动粒子的计算提取,对齐和平均值.
  • 实现了完整的鞭毛电机的高分辨率 (7纳米) 3D重建.

主要成果:

  • 完整的鞭毛电机的现场结构在7nm分辨率下得到解析.
  • 首次可视化了具有16倍对称性的定位器组件.
  • 观察到状态器直接连接到转子,C环,以及一种新的P环状结构.
  • 电机的大尺寸表明了增强扭矩产生机制.

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Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
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Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Published on: March 10, 2020

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Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

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结论:

  • 这项研究提供了第一个完整的细菌鞭毛电机的现场结构视图.
  • 这些发现揭示了定位器的对称性及其与其他电机组件的直接连接.
  • 结果支持扭矩产生模型,其中涉及C环的相互作用,FliG的特定域位于C环.