基因类线粒体中的RNA编辑模型:"指导"RNA分子从最大圆DNA转录,提供编辑的信息
B Blum1, N Bakalara, L Simpson
1Department of Biology, University of California, Los Angeles 90024.
Cell
|January 26, 1990
概括
小型RNA分子,称为指导RNA (gRNA),在Leishmania tarentolae中,线粒体可以编排RNA编辑. 这些gRNA通过混合形成和拟议的编辑周期指导精确的mRNA修改.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 寄生虫学的寄生虫学
背景情况:
- 线粒体RNA编辑对于某些生物体的基因表达至关重要.
- 莱什曼尼亚tarentolae在其线粒体转录中表现出广泛的RNA编辑.
研究的目的:
- 为了研究小RNA分子在Leishmania tarentolae中所扮演的角色.
- 阐明这些小RNAs促进mRNA成熟的机制.
主要方法:
- 对编码小RNA的线粒体DNA序列的分析.
- 小RNA序列与预编辑和成熟的mRNA序列的比较.
- 模拟RNA-RNA相互作用和编辑过程.
主要成果:
- 在Leishmania tarentolae线粒体中发现了一类小RNA (指导RNA).
- gRNAs具有与成熟mRNAs编辑区域相补充的序列.
- gRNAs可以与预编辑的mRNAs在编辑的位置上游形成部分杂交.
结论:
- gRNAs可能参与指导线粒体RNA编辑在Leishmania.
- 一个模型提出了gRNA-mRNA杂交,启动了分裂,尿酸酸添加/删除和编辑的重组的循环.
- 这种机制解释了gRNA和成熟mRNA之间的精确序列互补性.
相关概念视频
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Editing
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...


