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Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
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激活内皮上的VCAM-1与白细胞整体蛋白VLA-4相互作用,该整体蛋白与VLA-4/纤维蛋白结合部位不同.

M J Elices1, L Osborn, Y Takada

  • 1Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.

Cell
|February 23, 1990
PubMed
概括
此摘要是机器生成的。

整体VLA-4作为血管细胞粘附分子-1 (VCAM-1) 的受体,调解淋巴细胞粘附. 这种相互作用对于将单核白细胞招募到炎症部位至关重要.

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科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 分子医学是分子医学.

背景情况:

  • 细胞因子激活的内皮细胞表达血管细胞粘附分子-1 (VCAM-1).
  • VCAM-1 在淋巴细胞结合中起作用.
  • 了解VCAM-1受体是免疫细胞贩运的关键.

研究的目的:

  • 在淋巴细胞上识别VCAM-1的特定受体.
  • 阐明调控VCAM-1-介导细胞粘附的分子相互作用.
  • 为了区分VLA-4与VCAM-1和纤维菌素的相互作用.

主要方法:

  • 用VLA-4α4亚单元cDNA感染K-562细胞.
  • 评估细胞对VCAM-1和纤维菌素的粘附性.
  • 在抑制研究中使用抗VLA-4抗体和可溶性纤维素片段.

主要成果:

  • 鉴定出VLA-4 (Very Late Antigen-4) 是VCAM-1的特定受体.
  • 在K-562细胞上的VLA-4表达调节了对VCAM-1的特异粘附.
  • 此外,VLA-4还调节了纤维菌素对肝素II结合区域 (FN-40) 的粘附.
  • 通过特定的抑制剂来区分VLA-4/VCAM-1和VLA-4/纤维素蛋白相互作用.

结论:

  • 确定了VLA-4/VCAM-1连接体-受体对.
  • 这种相互作用对单核白细胞向炎症部位的招募具有重要意义.
  • VLA-4对VCAM-1和纤维菌素具有明显的结合特性.