转基因小鼠中的视网母细胞瘤
J J Windle1, D M Albert, J M O'Brien
1Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
Nature
|February 15, 1990
概括
研究人员创建了一个小鼠模型用于视网膜母细胞瘤,儿童眼睛癌症. 将特定的病毒瘤基因引入小鼠视网膜导致与人类视网膜母细胞瘤相同的瘤,提供了一个新的研究工具.
科学领域:
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
- 眼科医生 眼科 眼科
背景情况:
- 视网母细胞瘤是一种儿童眼部癌症,与视网母细胞瘤基因 (Rb) 的突变有关.
- 该Rb基因编码了p105Rb蛋白,对于瘤抑制至关重要.
- 了解视网母细胞瘤的遗传基础是癌症研究的关键.
研究的目的:
- 开发一种新的动物模型来研究视网膜母细胞瘤.
- 研究病毒瘤基因在视网膜母细胞瘤发育中的作用.
- 在体内探索病毒coproteins和Rb蛋白之间的相互作用.
主要方法:
- 生产转基因小鼠,具有病毒40 T抗原的眼球特异表达.
- 诱导眼瘤的组织学,超结构和免疫组织化学分析.
- 在瘤细胞中检查p105Rb和T抗原之间的关联.
主要成果:
- 转基因小鼠开发了可遗传的眼瘤,反映了人类视网膜母细胞瘤.
- 瘤表现出与人类视网膜母细胞瘤相同的组织学,超结构和免疫组织化学特征.
- 在小鼠瘤细胞中发现了p105Rb和T抗原之间的特定关联.
结论:
- 一种Rb结合性coprotein的眼球特异表达可以驱动视网膜母细胞瘤瘤发生.
- 这种小鼠模型提供了体内证据,证明Rb的功能失活是由病毒coproteins.
- 开发的小鼠模型对于研究视网膜母细胞瘤的发病和治疗策略非常有价值.
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