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在Drosophila发育过程中E74转录的空间和时间模式

C S Thummel¹, K C Burtis, D S Hogness

¹Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.

|April 6, 1990

概括

这是E74基因.

科学领域:

分子生物学分子生物学
发育生物学发展生物学
遗传学是一个遗传学.

背景情况:

E74基因是Drosophila. ecdysone调节的膨胀模式的关键组成部分.
阿什伯纳模型描述了昆虫发育中的埃克迪松诱导的基因表达动态.

研究的目的:

为了研究由ecdysone调节E74基因促进器.
检查E74A基因转录在Drosophila发育和时间中的作用.

主要方法:

对Ecdysone的反应中E74A促进剂活性的分析.
测量新生的E74A转录和气泡大小相关性.
在Drosophila发育过程中对E74A和E74B转录模式的表征.

主要成果:

埃克迪松直接激活了E74A促进体,随后由诱导的蛋白质抑制.
在特定的发育阶段,E74A转录在各种组织中被诱导.
E74A基因充当定时器,将mRNA的出现延迟大约一个小时.
E74B转录表现出一个独特的时间模式,与E74A重叠.

结论:

这些发现支持并扩展了Ashburner模型,突出了ecdysone脉冲在协调组织发育中的作用.
E74基因的时间调节对于Drosophila的精确发育过渡至关重要.

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