在土壤中的氨氧化原核生物中,古生物占主导地位
S Leininger1, T Urich, M Schloter
1Department of Biology, University of Bergen, Jahnebakken 5, N-5020 Bergen, Norway.
Nature
|August 18, 2006
概括
古代氨氧化剂在土壤中比细菌氧化剂要丰富得多,在循环中起着至关重要的作用. 这一发现突显了它们在土壤生态系统和氨氧化过程中的支配地位.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物地质化学生物地质化学
背景情况:
- 氨氧化启动了化,这是全球循环中的一个重要过程.
- 酸盐的可用性会影响植物营养,并可能导致地下水通过漏而受到污染.
研究的目的:
- 为了研究土壤生态系统中古代氨氧化剂的丰富性.
- 为了比较古代氨氧化剂的丰富性与它们的细菌对应物.
主要方法:
- 在12种土壤中研究了氨一氧化酶 (amoA) 基因的丰富性.
- 使用Crenarchaeota特定的脂质 (例如,crenarchaeol) 作为生物标志物.
- 雇佣的逆转录定量PCR和火烧测序用于活动和丰度分析.
主要成果:
- 考古阿莫A基因拷贝比细菌阿莫A基因多达3000倍.
- 丰富的古老阿莫A基因与Crenarchaeota特定的脂质相关.
- 证明了古生物的 in situ 活动,证实了它们的数量优势.
结论:
- 古代氨氧化剂在土壤生态系统中数量占主导地位.
- 在陆地环境中,Crenarchaeota可能是主要的氨氧化生物.
- 这对理解循环和土壤微生物生态学有重大影响.
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