1-Butyryl-glycerol:一种通过分化脂肪细胞分泌的新型血管生成因子
1Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|April 30, 1990
概括
脂肪细胞分泌monobutyrin,这是一种显著刺激血管生成 (新血管形成) 和内皮细胞运动性的分子. 这一发现揭示了单丁作为正常组织发育和血管生成的关键调节剂.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 生理学 生理学 生理学
背景情况:
- 脂肪细胞分化与促进血管生成和内皮细胞行为的因素的分泌有关.
- 现有知识表明脂肪细胞功能和血管发育之间存在联系,但特定的分子介质尚未完全阐明.
研究的目的:
- 识别和表征通过分化脂肪细胞分泌的特定分子,这些分子刺激血管生成和内皮细胞运动.
- 研究这些分子在正常细胞和组织发育的背景下所起的作用.
主要方法:
- 脂肪细胞受条件介质的分解,以分离不同的生物活动.
- 气色谱-质谱 (GC-MS) 用于分子识别.
- 在体内小胆质膜测试以评估血管生成潜力.
- 在体外测试以评估内皮细胞的增殖和运动.
主要成果:
- 血管新生和动力刺激活动是可以从内皮细胞中分离出来的.
- 鉴定出1-丁糖醇 (单丁) 是主要的依赖于分化的血管生成分子.
- 在脂肪细胞分化过程中,单丁的水平增加了200倍以上.
- 合成的单聚氨酸复制了血管和内皮细胞运动活性刺激作用,而不会影响增殖.
- 血管新生被刺激在非常低的剂量 (20 pg) 在小chorioallantoic膜测定.
结论:
- 单氨酸是参与脂肪细胞驱动血管生成的关键调节分子.
- 这一发现突显了单丁在与正常细胞和组织发育相关的过程中的作用.
- 莫诺布林特别促进血管新生和内皮细胞运动性,与线粒体效应不同.
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