不变链影响MHCII类分子的免疫学识别
1Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Nature
|May 10, 1990
概括
不变链 (Ii) 影响细胞如何向T细胞呈现抗原. 没有II,细胞显示改变的MHCII类分子,影响T细胞识别和抗体结合.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 细胞内事件对于形成由CD4+T细胞识别的MHCII类复合体至关重要.
- 不变链 (Ii) 是一种与MHCII类分子细胞内相关的非多态糖蛋白.
- Ii在调节加工抗原与MHCII类之间的相互作用中的作用正在调查中.
研究的目的:
- 调查不变链 (Ii) 在MHCII类复合体的形成和呈现中的作用.
- 确定II的存在是否会影响MHCII类分子对抗原的处理和呈现.
主要方法:
- 转染的纤维细胞细胞系的生成表达MHCII类与没有II.
- 分析这些细胞系的抗原处理和呈现能力.
- 使用T细胞测定和单克隆抗体结合的细胞表面MHCII类构造的表征.
主要成果:
- Ii的存在没有影响细胞处理和呈现完整抗原的能力.
- 阴性II细胞在细胞表面表现出MHCII类的变形.
- 这种在II阴性细胞中改变的MHCII类构造导致了对T细胞的抗原的呈现增加.
- Ii阴性细胞显示了几个II类特异性单克隆抗体的结合减少.
结论:
- 不变链 (Ii) 在调节MHCII类分子的构造方面发挥着重要作用.
- Ii通过影响MHCII类结构来影响抗原对T细胞的呈现.
- 细胞表面MHCII类的形状对T细胞识别和抗体结合至关重要.
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