来自细胞膜的共振增强性性SHG的独特对比模式
Ping Yan1, Andrew C Millard, Meide Wei
1Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
Journal of the American Chemical Society
|August 24, 2006
概括
合二元染料为细胞膜成像提供了新的非线性光学效果. 奇拉性破坏中心对称性,使得在细胞结处产生更强的第二子 (SHG) 显微镜信号.
科学领域:
- 非线性光学是一种非线性光学.
- 生物光子学 生物光子学
- 细胞成像 细胞成像
背景情况:
- 奇拉性可以诱导独特的非线性光学特性.
- 电压敏感染料对于细胞成像至关重要.
- 第二子 (SHG) 显微镜是一种强大的无标签成像技术.
研究的目的:
- 为 SHG 显微镜开发和描述一种新的性染色体.
- 为了研究性对活细胞中SHG信号的影响.
- 探索奇拉染料作为成像对比剂的潜力.
主要方法:
- 从电压敏感染料 (单体1) 中合成一种新性染色体 (二元体2).
- 活细胞的SHG显微镜染色与单体1,种族二次体,和奇拉二次体2.
- 在细胞膜和结点上对SHG信号进行比较分析.
主要成果:
- 所有染料选择性地染色细胞膜,产生强烈的SHG信号.
- 性消除了在中心对称细胞结处的SHG信号抑制.
- 在球形细胞的图像中,由于膜卷积,状二极体SHG破坏了极化模式.
结论:
- 状染色体可以通过破坏局部中心对称来增强SHG信号.
- 合二元染料显示出作为细胞成像的先进对比剂的潜力.
- 奇拉性影响SHG信号极化,提供了新的成像可能性.
相关概念视频
01:26Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
01:37Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.