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相关概念视频

The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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相关实验视频

Updated: May 10, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 30, 2010

RecA在转换中起作用,允许DNA聚合酶V通过DNA聚合酶V复制受损的DNA.

Katharina Schlacher1, Michael M Cox, Roger Woodgate

  • 1Department of Biological Sciences and Chemistry, University of Southern California, University Park, Los Angeles, California 90089-2910, USA.

Nature
|August 25, 2006
PubMed
概括
此摘要是机器生成的。

DNA 聚合酶 V (pol V) 和 RecA 蛋白质促进 DNA 修复. 这项研究揭示了ReCA.

更多相关视频

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

相关实验视频

Last Updated: May 10, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 30, 2010

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • DNA聚合酶V (pol V) 和ReCA对于大肠杆菌的突变性转化合成至关重要.
  • 这一途径可以修复DNA损伤,但其由ReCA进行的调节一直受到争议.
  • 之前的模型假设ReCA在合成过程中结合模板DNA链.

研究的目的:

  • 为了研究ReCA结合位置在pol V介导转化合成中的作用.
  • 阐明SOS突变发生过程中ReCA-DNA相互作用的机制.

主要方法:

  • 在体外测试检查pol V活动.
  • 在单链DNA (ssDNA) 上对RECA核蛋白丝的形成进行分析.
  • 研究ReCA结合"转化"对polV合成的影响.

主要成果:

  • 聚乙烯催化转化合成需要ReCA结合到分离的ssDNA分子 ("在trans"),而不是模板链.
  • 转基因DNA上的3'-近接点ReCA丝末端对于刺激polV活动至关重要.
  • 聚V和ReCA在跨导线上的进一步相互作用增强了合成.

结论:

  • RecA通过与ReCA与分离的ssDNA分子结合的相互作用来刺激pol V活动.
  • 这种"转刺激"机制解决了ReCA在SOS突变发生中的作用悖论.
  • 提出了一个用于转化合成和突变的新型调节机制.