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相关概念视频

Proofreading01:43

Proofreading

Overview
Mismatch Repair01:36

Mismatch Repair

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Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

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相关实验视频

Updated: May 12, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

RNA编辑涉及在精确定义的编辑领域中不分青红白地进行U变化.

C J Decker1, B Sollner-Webb

  • 1Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Cell
|June 15, 1990
PubMed
概括
此摘要是机器生成的。

试类动物中的RNA编辑包括在线粒体转录中插入/删除U-残留物. 这项研究揭示了编辑在域内是不分青红白的,发生在多化之前,这表明了修改和保护的循环.

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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Published on: December 11, 2020

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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Last Updated: May 12, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 寄生虫学的寄生虫学

背景情况:

  • RNA编辑是Trypanosomatids的一个独特的转录后修饰.
  • 它涉及在线粒体转录中插入或删除 uracil (U) 残留物.
  • 了解RNA编辑的精确机制和调节至关重要.

研究的目的:

  • 为了研究RNA编辑特征在tripanosomatid线粒体转录中.
  • 用一种新的cDNA克隆方案分析部分编辑的COIII和CYbRNA.
  • 阐明编辑过程中U插入/删除的模式和潜在机制.

主要方法:

  • 使用了一种新的cDNA克隆策略.
  • 分析了部分编辑的线粒体转录,特别是COIII和CYbRNAs.
  • 检查了编辑领域内的U-残留修改的分布和性质.

主要成果:

  • 在特定的编辑领域内,RNA编辑是不分青红白的,影响必要和不必要的网站.
  • 不完整的编辑仅限于精确的域名,不扩展到相邻的未编辑区域.
  • 编辑过程似乎在转录的多化之前开始.
  • 提出了一个模型,涉及裂变,U添加/删除和重组的循环,并保护正确编辑的网站.

结论:

  • 试类动物中的RNA编辑是一种复杂的过程,其特征在于在定义的领域内不分青红白的U-残留物修饰.
  • 编辑的时间相对于多基化和拟议的保护机制提供了对RNA处理的新见解.
  • 需要进一步的研究,才能充分了解这种独特的RNA修饰系统中涉及的酶和调节因素.