相关实验视频
Updated: Jul 11, 2026
08:23
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
Published on: December 28, 2011
转录基因组学将FOX转录因子与人类心力衰竭联系在一起
Sridhar Hannenhalli1, Mary E Putt, Joan M Gilmore
1Department of Genetics and Penn Center for Bioinformatics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
Circulation
|September 6, 2006
概括
在小鼠模型中发现的特定转录因子 (TF) 与人类心力衰竭有关. 这项研究突出了FOX家族TFs在人类心力衰竭中的作用,在动物模型和人类疾病之间提供了桥梁.
科学领域:
- 心血管生物学 心血管生物学
- 分子遗传学 分子遗传学
- 基因组学就是基因组学.
背景情况:
- 转录因子 (TFs) 在小鼠心力衰竭模型中调节心脏基因表达.
- 这些TF在人类心力衰竭中的相关性以前没有被研究过.
研究的目的:
- 用计算方法测试特定心脏TF与人类心力衰竭相关的假设.
- 为了确定参与人类心力衰竭病变的新型TF.
主要方法:
- 转录基因组学应用于缺陷 (n=196) 和非缺陷 (n=16) 人类心脏的RNA分离物.
- 分析不同表达基因的促进子区域的TF结合位点.
- 与在小鼠心力衰竭模型中发现的TF进行比较.
主要成果:
- 在人类心力衰竭基因中,MEF2,NKX,NF-AT和GATA TFs的结合点显著过多 (P<0.002).
- 在人类和小鼠心力衰竭 (P<0.002) 中,FOX TF结合部位过多.
- 表达式分析证实了FOXC1,C2,P1,P4和O1A在失败的人类心脏肌细胞和FOXP1在细胞核中.
结论:
- 提供了第一个将MEF2,NKX,NFAT和GATA TF与人类心力衰竭联系起来的证据.
- 在心力衰竭的发病过程中涉及FOX家族TFs (FOXC1,C2,P1,P4,O1A).
- 建立了动物模型和人类心力衰竭之间的联系,表明FOX信号在心脏缩应激反应中的作用.
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