相关实验视频
Updated: Jul 14, 2026
10:37
Spatial Separation of Molecular Conformers and Clusters
Published on: January 9, 2014
从气相离子/离子电子转移中通过气相离子/离子电子转移的质子聚基形成的离子/分子反应
Yu Xia1, Paul A Chrisman, Sharon J Pitteri
1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.
Journal of the American Chemical Society
|September 7, 2006
概括
多重质子聚酸离子基与氧反应,形成添加物,在激活时碎片化. 这种氧气反应有助于区分片离子,以改善蛋白质分析.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 物理化学 物理化学
背景情况:
- 多个质子聚酸通过气相电子转移形成离子基.
- 电子转移解离 (ETD) 对于和蛋白质主要结构的表征至关重要.
- 阴极是ETD中的反应性中间体.
研究的目的:
- 研究聚酸离子基与分子氧的反应.
- 探索这些反应对于区分片离子的有用性.
- 确定离子/基因反应是否可以探测基因位点位置.
主要方法:
- 气相电子转移到多重质子聚.
- 离子基与电动离子陷中的分子氧的反应.
- 氧气添加物的碰撞激活.
- 分离产物的分析. 分离产物的分析.
主要成果:
- 离子基很容易在室温下附着分子氧 (O(2) .
- 在碰撞激活时,氧气添加碎片,失去O{2),HO{*),或HO{2)).
- 碎片离子 (z-离子) 与O2反应,使其与c-离子区别开来.
- 完整的电子转移产物也形成具有类似解离行为的氧添加物.
结论:
- 与氧气的离子/基因反应可以区分c型和z型片离子.
- 氧的附着和随后的解离可以探测电子转移产品中的基因位点位置.
- 这种反应性为使用质谱学进行蛋白质识别和表征提供了一种新的方法.
相关概念视频
01:19Chemical Reactions
A chemical reaction is a process by which the bonds in the atoms of substances are rearranged to generate new substances. Matter cannot be created or destroyed in a chemical reaction—the same type and number of atoms that make up the reactants are still present in the products. Merely, the rearrangement of chemical bonds produces new compounds.
Chemical Reactions Rearrange Atoms into New Substances
A chemical reaction takes starting materials—the reactants—and changes them into different...
Chemical Reactions Rearrange Atoms into New Substances
A chemical reaction takes starting materials—the reactants—and changes them into different...
03:06Reaction Mechanisms
Chemical reactions often occur in a stepwise fashion, involving two or more distinct reactions taking place in a sequence. A balanced equation indicates the reacting species and the product species, but it reveals no details about how the reaction occurs at the molecular level. The reaction mechanism (or reaction path) provides details regarding the precise, step-by-step process by which a reaction occurs.
For instance, the decomposition of ozone appears to follow a mechanism with two steps:
For instance, the decomposition of ozone appears to follow a mechanism with two steps:
02:26Chemical Reactions
A balanced chemical equation provides the information of chemical formulas of the reactants and products involved in the chemical change. A reaction’s stoichiometry helps predict how much of the reactant is needed to produce the desired amount of product, or in some cases, how much product will be formed from a specific amount of the reactant.
The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in...
The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in...
02:13Energy Diagrams, Transition States, and Intermediates
Free-energy diagrams, or reaction coordinate diagrams, are graphs showing the energy changes that occur during a chemical reaction. The reaction coordinate represented on the horizontal axis shows how far the reaction has progressed structurally. Positions along the x-axis close to the reactants have structures resembling the reactants, while positions close to the products resemble the products. Peaks on the energy diagram represent stable structures with measurable lifetimes, while other...
01:08Types of Chemical Reactions: Exchange and Reversible
An exchange reaction is a chemical reaction in which both synthesis and decomposition occur, chemical bonds are both formed and broken, and chemical energy is absorbed, stored, and released.
A special kind of exchange reaction is the oxidation-reduction reaction, or the redox reaction. These reactions involve the transfer of electrons from one compound to another. The electrons in these reactions commonly come from hydrogen atoms, which consist of an electron and a proton. A molecule gives up a...
A special kind of exchange reaction is the oxidation-reduction reaction, or the redox reaction. These reactions involve the transfer of electrons from one compound to another. The electrons in these reactions commonly come from hydrogen atoms, which consist of an electron and a proton. A molecule gives up a...
01:17Ion Exchange
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...