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Updated: Jul 5, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
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改进的光稳定FRET-competent基于化光素的素四氨酸探针

Carla C Spagnuolo1, Rudolf J Vermeij, Elizabeth A Jares-Erijman

  • 1Departamento de Química Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria-Pabellón II, 1428, Buenos Aires, Argentina.

Journal of the American Chemical Society
|September 14, 2006
PubMed
概括

与原来的FlAsH相比,新的替代的双四氨酸标签 (F2FlAsH和F4FlAsH) 提供了优越的光稳定性,pH不敏感性和增强的光谱特性,使新的FRET对成为可能.

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...