大肠杆菌的bgl操作子中的转录抗终结是由一种特定的RNA结合蛋白调节的
F Houman1, M R Diaz-Torres, A Wright
1Department of Molecular Biology and Microbiology, Tufts University Health Sciences Campus, Boston, Massachusetts 02111.
Cell
|September 21, 1990
概括
大肠杆菌中的BglG蛋白与特定的RNA序列结合,防止转录终止. 这种RNA结合机制对于调节bgl操作子和确保基因表达至关重要.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 细菌的基因表达受到严格监管.
- 大肠杆菌中的bgl操作子控制着阿里尔-β-葡萄糖的新陈代谢.
- 监管涉及到在特定终结位点进行转录的反终结.
研究的目的:
- 研究BglG蛋白调节bgl操作子转录的机制.
- 为了确定RNA序列和BglG结合的结构要求.
- 阐明BglG结合如何防止转录终止.
主要方法:
- 测试Oligonucleotide来界定BglG识别序列的范围.
- 目标RNA序列的突变分析.
- RNA结合试验 (隐含的).
主要成果:
- BglG是一种RNA结合蛋白.
- 在转录终结器上游,BglG可以识别特定的序列.
- 特定的RNA二次结构对于BglG识别至关重要.
- BglG结合对于抗终结是必不可少的.
结论:
- BglG通过与新生的RNA结合来防止转录终止.
- 这种结合事件可能会干扰终端结构的形成.
- BglG-RNA相互作用是bgl操作子的一个关键调节机制.
相关概念视频
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Transcription Attenuation in Prokaryotes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Bacterial Transcription
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription in Prokaryotes
Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow box)...
Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...


