相关实验视频

Updated: May 5, 2026

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

套件连接体:一个细胞表面分子在钢铁突变纤维细胞中发生变化

J G Flanagan¹, P Leder

¹Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115.

|October 5, 1990

概括

研究人员确定了kit连接体,一种与c-kit受体相互作用的蛋白质. 这一发现证实了Kit联结体的存在.

科学领域:

分子生物学分子生物学
发育生物学发展生物学
细胞信号传递细胞信号传递

背景情况:

该c-kit原型瘤基因编码了一个细胞表面受体,具有未知的联结体.
了解c-kit联体对解读其在发育和疾病中的作用至关重要.
现有的方法缺乏识别和表征未知的配体的灵敏度.

研究的目的:

为了识别和表征c-kit受体的未知连接体.
开发一种用于检测和研究细胞表面受体连接体的新方法.
为了研究钢 (Sl) 位点在联体表达中的作用.

主要方法:

在基因上将c-kit受体的细胞外域与胎盘酸酶 (AP) 融合.
创建了一个可溶性受体亲和反应剂,APtag-KIT,用于连接体检测.
使用APtag-KIT进行3T3纤维细胞细胞系的结合试验和现场染色.

主要成果:

APtag-KIT 特别与3T3 纤维细胞上的一个连接体结合,其解离常数 (KD) 为3 x 10(-8) M.
在现场染色显示,该联体在3T3纤维细胞的细胞表面表达.
在具有钢 (Sl) 突变的3T3纤维细胞上取消了结合,表明Sl位部参与.

结论:

直接的分子证据证实了套件连接体存在于细胞表面蛋白质中.
钢 (Sl) 位点对于套件连接体的表达或结构至关重要.
这项研究提供了一种新的方法来识别和表征未知的受体连接体.

更多相关视频

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Published on: June 27, 2017

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

Published on: November 6, 2019

相关概念视频

Ligand Binding Sites

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...