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通过CD10/中性内皮酶降低对恩基法林介导的炎症反应的降低调节 24.11

M A Shipp¹, G B Stefano, L D'Adamio

¹Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.

|September 27, 1990

概括

酶CD10 (常见的急性淋巴细胞白血病抗原),也称为中性内酶24.11 (NEP),调节炎症反应. 这种古老的酶结构在各个物种中都保持着,影响血细胞激活和脑内介导的炎症.

科学领域:

免疫学免疫学免疫学
生物化学生物化学
进化生物学进化生物学

背景情况:

CD10 (常见的急性淋巴细胞白血病抗原),一种金属蛋白酶 (中性内皮酶24.11或NEP),在各种免疫细胞上发现,并水解脑细胞.
甲基核素诱导无脊椎动物血细胞的炎症反应,例如在软体动物Mytilus edulis.

研究的目的:

研究Mytilus edulis血细胞中CD10/NEP相关结构的存在和功能.
为了确定CD10/NEP是否在无脊椎动物和人类中调节脑胺介导的炎症反应中发挥作用.

主要方法:

在M. edulis血细胞中对CD10/NEP相关结构的表达分析.
酶活性测定用于评估CD10/NEP废除对Met-enkephalin诱导的血细胞激活的影响.
与人类多态核白细胞进行比较分析.

主要成果:

与CD10/NEP相关的结构是由M. edulis血细胞表达的.
取消CD10/NEP酶活性显著降低了血细胞激活所需的Met-enkephalin度.
在M. edulis和人类白细胞中观察到CD10/NEP在脑内介导炎症中的类似调节作用.

结论:

与CD10/NEP相关的结构在调节包括软体动物和人类在内的多种物种中脑介导的炎症反应方面具有功能性保护.
这表明CD10/NEP在免疫系统功能中的古代进化作用,可以追溯到5亿多年前.

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