细胞内疟疾寄生虫对无机酸盐的依赖的吸收
Kevin J Saliba1, Rowena E Martin, Angelika Bröer
1School of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia.
Nature
|September 29, 2006
概括
疟疾寄生虫通过它们的膜使用 (Na+) 梯度来吸收必需的无机酸盐 (P(i)). 这一发现揭示了Plasmodium falciparum中营养吸收的新机制.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 疟疾寄生虫 (Plasmodium falciparum) 感染增加了宿主红细胞对Na+和K+等溶液的透性.
- 这导致红细胞细胞质中Na+度更高,并通过寄生虫膜强烈向内Na+梯度.
研究的目的:
- 为了研究Plasmodium falciparum如何利用Na+梯度来吸收营养.
- 识别和描述负责无机酸盐 (P) 吸收的载体.
主要方法:
- 研究了细胞内寄生虫对无机酸盐 (P(i)) 的吸收.
- 克隆并鉴定了来自Plasmodium falciparum的PfPiT输送体.
- 在Xenopus卵细胞中表达PfPiT以确认功能.
主要成果:
- 菌吸收无机酸盐 (P(i)) 通过一个Na+依赖的载体.
- 输送器表现为2Na+:1P(i) 立体几何学,并且更喜欢单价P(i).
- 克隆的PfPiT载体,表达在卵细胞中,调解了具有类似特征的Na+依赖P (i) 吸收.
结论:
- 疟疾寄生虫利用宿主细胞的改变的离子环境来激活无机酸盐 (P) 的吸收.
- PfPiT是一种关键的寄生虫表面载体,参与营养获取.
- 这突显了寄生虫适应操纵宿主细胞生理学的适应性,以实现其生长.
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