一个核酸附加的形的分子级螺旋堆 ((p-phenylenevinylene) 通过超分子自组装以补充的寡核酸作为模板的指导
Rika Iwaura1, Freek J M Hoeben, Mitsutoshi Masuda
1Nanoarchitectonics Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5, Tsukuba, Ibaraki 305-8565, Japan.
Journal of the American Chemical Society
|October 5, 2006
概括
核酸附加的基烯 (p-phenylenevinylene) 自组装成螺旋结构. 二元组合与互补的DNA模板形成右侧螺旋状堆,由胺-氨酸基配对驱动.
科学领域:
- 超分子化学 超分子化学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 奥利戈 ((p-phenylenevinylene) 是一种结合聚合物,在电子和光子学中具有潜在的应用.
- 核酸附加聚合物提供了一条将合成聚合物的特性与核酸的特定识别能力相结合的途径.
- 自组装是从分子构建块创建有序纳米结构的关键过程.
研究的目的:
- 为了合成一个核酸附着的奥利戈 (p-phenylenevinylene) (8).
- 为了研究化合物8的自我组装行为,无论是作为单个成分还是与DNA链的二元混合物.
- 探索由DNA模板引发的有序纳米结构,特别是螺旋堆的形成.
主要方法:
- 合成核酸附加的奥利戈 (p-烯乙烯) (8).
- 原子力显微镜 (AFM) 可视化自组装结构.
- 紫外线可见 (UV-vis) 和循环二极化 (CD) 光谱分析分子组织和性.
主要成果:
- 8个单元自组装没有产生显著的纤维结构.
- 8个与补充的20-meric oligodeoxyadenylic acid (9) 的二进制自组装导致了右转螺旋堆 (直径为6.4和5.1纳米).
- 螺旋堆的形成取决于氨酸 (T) 和氨酸 (A) (1:1和2:1的比例),表明T-A基配对是驱动力.
结论:
- 8的核酸部分和DNA模板 (9) 之间的互补的T-A基配对诱导螺旋性形形形的形成.
- DNA模板的度强烈影响CD光谱,证实其在指导组装中的作用.
- 非互补的DNA (寡胺基酸,10) 并没有诱导有序螺旋结构的形成,突出了模板过程的特异性.
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