通过受体氨酸激酶进行核信号传递:春天的第一个红
Joseph Schlessinger1, Mark A Lemmon
1Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA. joseph.schlessinger@yale.edu
Cell
|October 5, 2006
概括
受体氨酸激酶 (RTK) 可以在核内发出信号. 一个ErbB4受体片段移动到核中,形成一个抑制星细胞促进基因转录的复合体.
科学领域:
- 蜂信号传输是如何进行的
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 受体氨酸激酶 (RTKs) 是跨膜信号的关键.
- RTKs的核功能,特别是依赖连接体的转位,尚未完全理解.
- ErbB4是一种RTK,涉及各种细胞过程.
研究的目的:
- 为了研究细胞内ErbB4受体碎片在细胞核中的功能意义.
- 阐明核ErbB4信号传输的分子机制.
- 确定核ErbB4信号对基因表达的影响,特别是在星球细胞形成中.
主要方法:
- 激发ErbB4受体的连接体.
- 对受体片段转移到核的分析.
- 同免疫沉用于识别蛋白质复合体 (ErbB4片段,TAB2,N-CoR).
- 使用RT-qPCR等技术对核中的基因表达变化的定量分析.
- 记者测试以评估转录压制.
主要成果:
- 干激活释放了一个细胞内ErbB4受体片段.
- 这个碎片转移到核中.
- ErbB4片段与适配器TAB2和核心压缩器N-CoR组成了一个功能复合体.
- 核复合体抑制了参与天体细胞形成的基因的转录.
结论:
- 细胞内ErbB4受体片段可以参与直接的核信号传输.
- 通过与TAB2和N-CoR的复合体介导的核ErbB4信号传输,在转录抑制中发挥作用.
- 这种核信号传递途径影响与天体细胞发育相关的基因表达,这表明RTKs在细胞膜之外发挥了新的作用.
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