两性 (AAKA) n的聚合成反平行β片
Thomas J Measey1, Reinhard Schweitzer-Stenner
1Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA.
Journal of the American Chemical Society
|October 13, 2006
概括
旨在形成螺旋的可以惊人地自组装成具有β-sheet结构的基. 这种意想不到的β片形成甚至发生在带有正电荷的氨酸残留物中,挑战了典型的类自我组装预期.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 超分子化学 超分子化学
背景情况:
- 据预测,带有两性病重复单元 (Ac-(AAKA) n-NH2) 的可以形成螺旋结构.
- 螺旋轮的投影表明这些Ac-(AAKA) n-NH2的两性性质.
- 预计较高的残留量通常有利于水溶液中螺旋形形成.
研究的目的:
- 为了研究Ac-(AAKA) n-NH2在水溶液中的自我组装行为.
- 确定这些在不同度下采用的二次结构.
- 了解控制体自我组装成更高阶结构的因素.
主要方法:
- 循环二元化 (ECD) 光谱法用于评估二次结构.
- 类度的变化是为了观察对自我组装的影响.
- 在宏观上观察到水凝的形成.
主要成果:
- 在毫米克度下,Ac-(AAKA) 4-NH2和Ac-(AAKA) 3-NH2体显示了一些螺旋性含量.
- 令人惊的是,在厘米度下,这些酸形成水凝.
- 水凝的特点是反平行β-sheet形状,与螺旋式的预期相反.
结论:
- 两性Ac-(AAKA) n-NH2可以采用反平行β片形状.
- 这些可以在更高的度下自组合成水凝,覆盖螺旋倾向.
- 由于存在充电的氨酸残留物,β-sheet结构的形成是意想不到的.
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