由HIV-1的超变中和决定因素引起的广泛中和抗体
K Javaherian1, A J Langlois, G J LaRosa
1Department of Surgery, Duke University Medical School, Durham, NC 27710.
Science (New York, N.Y.)
|December 14, 1990
概括
针对V3循环的抗体
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 人类免疫缺陷病毒 (HIV) -1的主要中和决定因素 (PND) 位于包膜蛋白的V3循环中.
- 针对该区域产生的抗体可以中和各种HIV-1分离物.
研究的目的:
- 为了研究由特定的V3环引起的抗体的交叉中和潜力.
- 在PND中识别保护的氨基酸序列,负责交叉中和.
主要方法:
- 用代表HIV-1 PND的合成体免疫动物.
- 中和测试使用来自免疫动物的血清对抗各种HIV-1分离体.
- 抗体与特定的氨基酸序列结合的特征.
主要成果:
- 来自用HTLV-IIIMN PND (RP142) 免疫动物的血清中和了MN和HTLV-IIIB分离物.
- 来自用IIIB PND (RP337) 免疫动物的血清也中和了这两种分离物.
- 交叉中和抗体识别了GlyProGlyArgAlaPhe (GPGRAF) 序列,这种序列在分离物中发现,并且经常在其他PND中发现.
结论:
- 在HIV-1 V3循环中的GPGRAF序列是交叉中和抗体的关键目标.
- 含有GPGRAF序列的可以引起抗体,这些抗体可以中和多种HIV-1分离物,包括HTLV-IIIB和MN.
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