在核糖体上传递 RNA 运动的结构基础
Gulnara Yusupova1, Lasse Jenner, Bernard Rees
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch cedex, France.
Nature
|October 20, 2006
概括
传递 RNA (mRNA) 在核糖体上的定位对于蛋白质合成至关重要. 这项研究揭示了mRNA在翻译启动和延伸过程中如何与核糖体相互作用,突出了不同的结合机制.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 基因表达水平受到翻译启动的显著影响.
- 在核糖体上精确定位信使RNA (mRNA) 对于产生功能性蛋白质至关重要.
- 在翻译过程中控制mRNA-核糖体相互作用的分子机制仍然不完全理解.
研究的目的:
- 阐明不同翻译状态的mRNA-核糖体相互作用的结构基础.
- 为了比较mRNA在启动,启动后和延长过程中的结合动态.
- 调查Shine-Dalgarno (SD) 双重体在mRNA定和运动中的作用.
主要方法:
- 对八个X射线晶体结构的核糖体复合体进行比较分析.
- 翻译启动,后启动和延伸状态的建模.
- 对mRNA与核糖体相互作用的结构比较.
主要成果:
- 在启动和启动后,Shine-Dalgarno (SD) 双重组在启动和后启动期间,将mRNA的5'端强烈定在30S核糖体亚单元平台上.
- 缺乏SD相互作用的mRNA在延长过程中在5'端表现出灵活性,这表明不同的退出途径.
- 在启动后,mRNA经历3'到5'运动,旋转和SD双重延长,导致与核糖体蛋白S2接触.
结论:
- 在翻译启动和延伸过程中,不同的结构机制控制着mRNA-核糖体相互作用.
- 在早期翻译阶段,SD相互作用在稳定mRNA方面发挥着关键作用.
- 核糖体-mRNA动态是复杂的,并且根据转化状态有很大差异.
相关概念视频
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...


