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相关概念视频

Protein Folding01:22

Protein Folding

Overview
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...

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相关实验视频

Updated: Jul 15, 2026

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Published on: March 29, 2010

C3b的结构揭示了构造变化,这些变化是补充活动的基础.

Bert J C Janssen1, Agni Christodoulidou, Andrew McCarthy

  • 1Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Nature
|October 20, 2006
PubMed
概括

补充系统是补充系统.

科学领域:

  • 免疫学 免疫学 免疫学
  • 结构生物学 结构生物学
  • 生物化学 生物化学

背景情况:

  • 补体系统对先天性和适应性免疫非常重要,有助于抵抗感染和清除细胞碎片.
  • 激活补充蛋白3 (C3b) 协同附着于表面,放大免疫反应,并标记细胞进行细胞分裂.
  • 对C3转变为C3b的形状变化,暴露其结合点,仍然在很大程度上是未知的.

研究的目的:

  • 阐明了补充蛋白3 (C3) 在C3b.中进行蛋白质分解激活所涉及的构造变化.
  • 为了呈现激活C3b的晶体结构和细节域重新排列.
  • 了解C3b如何暴露其蛋白质和受体相互作用的结合部位.

主要方法:

  • 在4-Å分辨率下确定激活补充蛋白C3b的晶体结构.
  • 分析了C3b的12个域在蛋白质溶解激活时的形状重排.
  • 将激活C3b的结构与原生C3进行比较,以确定关键变化.

主要成果:

  • 晶体结构显示C3b与原生C3.3相比存在显著的域重组.
  • 在C3b中的铁位完全暴露于共价附着,这与C3.3中的埋藏位置有显著的变化.
  • 改变的分子表面暴露了神秘的部位,与B因子和补充调节者的结合部位一致.

更多相关视频

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
06:54

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

相关实验视频

Last Updated: Jul 15, 2026

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Published on: March 29, 2010

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
06:54

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

结论:

  • 蛋白质分解性激活C3到C3b主要涉及主要在初始转化阶段的重大形状变化.
  • 这些对C3b形成的结构洞察力揭示了它的功能结合点是如何暴露的.
  • 了解这些结构动态对于开发用于补充介导炎症疾病的治疗策略至关重要.