相关实验视频
Updated: Jul 12, 2026

07:24
A Protocol for the Production of KLRG1 Tetramer
Published on: January 12, 2010
套件连接体生长因子的跨膜形式通过替代拼接确定,并且在Sld突变体中缺失
J G Flanagan1, D C Chan, P Leder
1Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|March 8, 1991
概括
组织特异性替代拼接产生两种形式的干细胞因子 (SCF),一种是可溶性形式和一种是细胞表面形式. 细胞表面SCF对于细胞粘附和生物体发育至关重要.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 对于c-kit受体的连接体 (KL),也称为干细胞因子 (SCF),是一种在小鼠钢 (Sl) 位点编码的生长因子.
- SCF存在于细胞表面和可溶性形式,但它们的调节和功能意义尚未得到充分理解.
研究的目的:
- 研究替代拼接在产生不同形式的SCF中的作用.
- 确定细胞表面与可溶性SCF在细胞过程和生物体发育中的功能意义.
主要方法:
- 使用特定组织的替代拼接对KL/SCFmRNA变异的分析.
- 对具有不同KL/SCFcDNA结构的细胞进行传染,以研究蛋白质表达和功能.
- 鉴定SId等位基因的特征,该等位基因是KL/SCF基因中的删除突变.
主要成果:
- 通过替代拼接产生两种类型的KL/SCFmRNA,从而产生不同的蛋白质形式.
- 一种mRNA变异优先产生可溶性SCF,而另一种则产生细胞表面SCF.
- 细胞表面SCF促进细胞增殖和细胞-细胞粘附.
- SId等位基因导致可溶性SCF缺乏细胞表面协会,这会影响造血细胞,黑色细胞和生殖细胞的发育.
结论:
- KL/SCF mRNA的替代拼接产生了功能上不同的蛋白质异型.
- 细胞表面SCF在细胞粘附中起着至关重要的作用,对于正常的生物体发育至关重要,特别是造血细胞,黑色细胞和胚胎细胞.
相关概念视频
Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Receptor Tyrosine Kinases
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

