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在植物中,siRNA生物发生的双击触发器
Michael J Axtell1, Calvin Jan, Ramya Rajagopalan
1Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Cell
|November 4, 2006
概括
双重的微RNA结合点,并不总是涉及裂变,触发了植物中小干扰RNA (siRNA) 生物发生的阶段性. 这种保存机制影响RNA沉默和识别.
科学领域:
- 植物分子生物学 植物分子生物学
- 在RNA沉默路径上.
- 表观遗传学和基因调节
背景情况:
- 微RNAs (miRNAs) 启动RNA指导的分裂,有时导致Arabidopsis中分阶段的小干扰RNAs (siRNAs).
- 大多数miRNA点不会产生siRNA,这表明其他因素参与siRNA生成.
研究的目的:
- 调查超出简单miRNA裂变的分相siRNA生物发生的决定因素.
- 在保存的siRNA位置中探索双miRNA结合位点的作用.
主要方法:
- 在和Arabidopsis中siRNA生成的比较分析.
- 在体外结合测定miR390和补充部位.
- 使用遗传方法对Arabidopsis中miR390补充位点的功能分析.
主要成果:
- 中的分相siRNAs来源于与双miR390分裂部位相邻的区域.
- 保存的siRNA位点AtTAS3具有双重miR390互补位点,两者在Arabidopsis中都是功能性的.
- 在植物中,不可切割的miRNA结合部位可以具有功能意义.
- 双重miRNA/siRNA互补位点,不一定是两个分裂事件,是siRNA生物发生的保存触发器.
结论:
- 一种涉及双miRNA结合点的保存机制,通常但不仅仅是通过裂变,启动分阶段siRNA生产.
- 这一发现对了解RNA识别和植物物种中异常RNA的沉默具有重要意义.
相关概念视频
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

