氨酸介导的RNA识别:氨酸叉
B J Calnan1, B Tidor, S Biancalana
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142.
概括
艾滋病毒-1 Tat 蛋白中的氨酸残留物特别结合于 TAR RNA 凸起,这对于交易激活至关重要. 这种阿尔金宁-RNA相互作用突出了阿尔金宁的作用.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 生物化学 生物化学
背景情况:
- 人类免疫缺陷病毒1型 (HIV-1) Tat蛋白对于病毒基因表达至关重要.
- 塔特蛋白与病毒RNA的交换活化反应 (TAR) 元素相互作用.
- 已知Tat的基本区域结合了TARRNA,但精确的分子相互作用尚未完全阐明.
研究的目的:
- 调查特定的氨基酸残留物,特别是氨酸在TAT衍生与TARRNA的结合中的作用.
- 确定这些相互作用对Tat蛋白的交换活化活动的贡献.
- 为了阐明RNA突起的阿尔金因介导识别的结构基础.
主要方法:
- 使用TAR RNA的合成和结合试验.
- 塔特蛋白的局部导向突变发生.
- 在体外的交换活化试验.
- 乙基化干扰实验. 干扰实验.
- 分子建模和结构分析.
主要成果:
- 一种含有九种阿尔金宁的 (R9) 显示出与TARRNA的特定结合.
- 一种突变的Tat蛋白与R9表现出完全的交换活化活动.
- 一种含有九种氨酸 (K9) 的,与 TAR 结合不良,相应的蛋白质表现出边际活性.
- 确定一个单一的阿基宁残留物,对特定的结合和交换活化至关重要.
- 乙基化干扰和建模表明,氨酸与RNA膨胀中的相邻酸盐接触.
结论:
- 氨酸侧链对于Tat与TARRNA突起的特定结合至关重要.
- 氨酸介导的结网与RNA酸盐可能有助于识别RNA结构动机.
- 氨酸可能是蛋白质用来识别特定RNA结构的常见残留物,特别是循环和凸起.
相关概念视频
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...


