Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Metal-Ligand Bonds02:51

Metal-Ligand Bonds

The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Single Photon Emission Computed Tomography Tracer.

Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer·2026
Same author

ATP synthase is a promising target for identifying activated and non-activated adipose tissues.

Nature communications·2026
Same author

The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study.

EJNMMI radiopharmacy and chemistry·2026
Same author

Validation of Folate Receptor-α Selectivity of 6<i>S</i>-5-Methyltetrahydrofolate-Based Radioconjugates: A Prerequisite for Therapeutic Application?

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
Same author

Targeted β<sup>-</sup>-Particle Plus Conversion and Auger-Electron Therapy with <sup>161</sup>Tb-Labeled Somatostatin Receptor Antagonist DOTA-LM3: A Phase 0 Study.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2025
Same author

First positronium lifetime imaging with scandium-44 on a long axial field-of-view PET/CT.

Frontiers in nuclear medicine·2025

相关实验视频

Updated: Jul 14, 2026

Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium
13:34

Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium

Published on: July 8, 2015

"点击合物":合成和安装金属合物到生物分子中的单个步骤.

Thomas L Mindt1, Harriet Struthers, Luc Brans

  • 1Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland.

Journal of the American Chemical Society
|November 23, 2006
PubMed
概括

研究人员利用点击化学来创建基于三醇的化系统,并将其附着在生物分子上. 这种"点击切拉"方法使生物分子的修饰能够用于诊断和治疗用途.

更多相关视频

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
09:44

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

相关实验视频

Last Updated: Jul 14, 2026

Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium
13:34

Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium

Published on: July 8, 2015

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
09:44

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

科学领域:

  • 药用化学 医学化学
  • 有机化学 有机化学
  • 生物结合的生物结合

背景情况:

  • 三醇化合物是药物化学中的多功能支架.
  • 金属合剂对于诊断成像和治疗至关重要.
  • 点击化学提供了高效可靠的结合方法.

研究的目的:

  • 开发基于三醇的新型多酸化系统.
  • 通过点击化学将这些合剂与生物分子结合起来.
  • 探索诊断和治疗中的应用.

主要方法:

  • 铜催化亚酸环添加 (CuAAC) 用于三醇的形成.
  • 合成多酸化联结体.
  • 化剂与生物相关分子的结合.
  • 由此产生的结合物的表征.

主要成果:

  • 成功组装了基于三醇的新结合系统.
  • 通过点击化学有效地将化剂附着在生物分子上.
  • 演示了该系统的性能.
  • 结论:点击化策略是用金属化剂修改生物分子的强大工具,为先进的诊断和治疗药物开辟了道路.

结论:

  • 点击化方法使金属化剂的有效生物结合成为可能.
  • 这种方法有助于开发新型诊断和治疗药物.
  • 基于triazole的化系统显示出生物医学应用的前景.