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基本和酸性纤维细胞生长因子在发育中的老鼠心脏中的免疫组织化学定位

P Spirito¹, Y M Fu, Z X Yu

¹Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md.

|July 1, 1991

概括

基本和酸性纤维细胞生长因子 (bFGF和aFGF) 在发育老鼠心脏中广泛分布,这表明它们在心脏发育和细胞分化中起着关键作用.

科学领域:

心血管生物学心血管生物学
发展生物学发展生物学
分子生物学分子生物学

背景情况:

基本纤维细胞生长因子 (bFGF) 和酸性纤维细胞生长因子 (aFGF) 是重要的信号分子.
它们在心脏发育中的作用需要详细的研究.

研究的目的:

研究bFGF和aFGF在发育中的老鼠心中的表达和分布.
为了将生长因子局部化与心脏组织发生和形态发生相关联.

主要方法:

采用了生物化学和免疫组织化学技术.
使用了西方斑点分析和基于抗体的免疫染色.

主要成果:

bFGF和aFGF (大约2个月) 18 kD) 在从胚胎第11天到产后第35天的老鼠心脏中显示重叠分布.
定位在细胞质和细胞外基质上,包括肌细胞,介质细胞和内皮细胞.
动脉光滑肌细胞的免疫抑制强度下降,与分化.

结论:

广泛的bFGF和aFGF表达表明它们在心脏细胞分化和形态发生过程中起着重要的作用.
叠加分布表明这些增长因素之间的潜在功能相互作用.
分布的动态变化意味着心脏器官发生过程中角色的演变.

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