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识别和扩展人类结肠癌发起细胞
Lucia Ricci-Vitiani1, Dario G Lombardi, Emanuela Pilozzi
1Department of Hematology and Oncology, Istituto Superiore di Sanità, Viale Regina Elena 299, Rome 00161, Italy.
Nature
|November 24, 2006
概括
结肠直肠癌是由一小群未分化的瘤原性CD133+细胞驱动的. 准这些特定的结肠癌干细胞为新型疗法提供了一个有希望的途径.
科学领域:
- 在瘤学瘤学.
- 癌症干细胞生物学
背景情况:
- 大肠直肠癌 (CRC) 是癌症相关死亡的主要原因.
- 识别致癌细胞对于开发有效治疗方法至关重要.
- 不分化细胞在CRC瘤发生中的作用需要进一步研究.
研究的目的:
- 隔离和表征结肠癌中的致瘤细胞.
- 为了确定特定的细胞群是否负责CRC启动和传播.
- 探索针对这些细胞进行治疗策略的潜力.
主要方法:
- 流细胞计测试用于识别和分离结肠瘤中的CD133+和CD133-细胞.
- 隔离细胞的皮下注射到免疫缺陷小鼠中,以评估瘤性.
- 连续移植瘤以评估长期的传播和稳定性.
- 在无血清介质中以瘤球体的形式培养细胞以评估自我更新和分化潜力.
主要成果:
- 在CD133+分数内确定了一个独特的瘤细胞群,约占瘤细胞的2.5%.
- 在体内,CD133+细胞很容易形成瘤,可以连续移植,而CD133-细胞则不能.
- CD133+结肠癌细胞在体外表现出指数增长,作为不分化的瘤球体,保持瘤发生潜力.
- 来自CD133+细胞的瘤在序列通道中显示出稳定的形态和抗原特征.
结论:
- 结肠直肠癌是由一小部分未分化的瘤原性CD133+细胞开始并维持的.
- 这些CD133+细胞作为结肠癌干细胞起作用.
- 向CD133+细胞是未来CRC治疗的有希望的策略.
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