聚类DNA基因标记X染色体以通过剂量补偿复合体进行抑制
Patrick McDonel1, Judith Jans, Brant K Peterson
1Howard Hughes Medical Institute, University of California-Berkeley, 16 Barker Hall, Berkeley, California 94720-3204, USA.
Nature
|November 24, 2006
概括
研究人员确定了特定的DNA序列动图,这些动图将剂量补偿复合体 (DCC) 招募到C. elegans的X染色体中. 这些常见动机的高密度共发生,而不是X染色体特定的序列,驱动着染色体范围内的基因抑制.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 基因表达调节发生在个体基因和大染色体域水平上.
- 控制大规模基因调节的cis作用信息机制仍然难以捉摸.
- 凯诺哈比迪斯的剂量补偿为全染色体基因抑制提供了一个模型.
研究的目的:
- 发现和描述针对X染色体进行由剂量补偿复合体 (DCC) 抑制的cis作用位.
- 阐明DNA序列在招募DCC中对全染色体基因调节的作用.
主要方法:
- 在C. elegans X染色体上识别和分析cis作用位 (rex位)
- 在雷克斯遗址内对特定DNA基因的突变分析.
- 在体内评估DCC与突变的雷克斯部位的结合.
主要成果:
- 在X染色体上发现广泛分散的雷克斯位点,这些位点位于促进体,表细胞和基因间区域.
- 在雷克斯地点内确定至少两个不同的动机,这对于DCC招聘至关重要.
- 证明高密度共发生的常见动机,而不是X特定序列,对于DCC绑定和压制至关重要.
结论:
- 主要DNA序列,特别是高密度的共同基因的共同发生,决定了X染色体针对DCC介导的基因抑制的向.
- 染色体范围的基因调节是通过非X特异性序列元素的分布来建立的.
- 这一发现促进了对表观遗传调节和基因剂量控制的理解.
相关概念视频
01:17Karyotyping
Overview
01:58X-Inactivation
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
02:50Dosage Compensation
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...