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多能胚胎isl1+原始细胞导致心脏,光滑肌肉和内皮细胞多样化
Alessandra Moretti1, Leslie Caron, Atsushi Nakano
1Massachusetts General Hospital - Cardiovascular Research Center, Charles River Plaza/CPZN 3208, 185 Cambridge Street, Boston, MA 02114, USA.
Cell
|November 25, 2006
概括
心血管发育涉及单一的多能原生细胞 (MICP),产生多种细胞类型. 这一发现为使用胚胎干细胞进行心血管组织再生提供了新的策略.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 心血管科学 心血管科学
背景情况:
- 传统上,心脏生成假定内皮细胞,心脏细胞和光滑肌肉细胞的明显胚胎前体.
- 了解心血管血统的细胞起源对于再生医学至关重要.
研究的目的:
- 在心血管细胞生成中研究来自第二心脏场的isl1(+) 前体的多效性.
- 使用胚胎干细胞识别和表征多能心血管原生细胞 (MICPs).
- 探索ES细胞衍生的MICPs在心血管组织再生方面的潜力.
主要方法:
- 在体内进行遗传命运映射研究,以追踪前体细胞系.
- 来自胚胎干细胞 (ES) 的心血管原始细胞的克隆放大.
- 转录分析,以确定多能心血管原始体的特征.
主要成果:
- 证明了来自第二个心脏场的前体可以产生内皮细胞,心脏细胞和光滑肌肉细胞.
- 通过转录特征定义的多能心血管原生细胞 (MICP) 被确定为isl1(+) /Nkx2.5(+) /flk1(+).
- 从类似胚胎前体的ES细胞中建立了来自ES细胞的1l1(+) 先生的细胞层次.
结论:
- 心血管系的多样化源于一个多能心血管原生细胞 (MICP) 的单细胞决定.
- 来自ES细胞的MICP代表了产生特定心血管细胞类型用于再生疗法的有希望的来源.
- 这项研究重新定义了心脏发生的发育范式,突出了多个心血管血统的共同祖先.
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