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Updated: Aug 4, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

P[acman]:一个BAC转基因平台,用于针对性地将大型DNA片段插入D. melanogaster中

Koen J T Venken1, Yuchun He, Roger A Hoskins

  • 1Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Science (New York, N.Y.)
|December 2, 2006
PubMed
概括

这项研究引入了一个新的Drosophila melanogaster转基因平台. 它可以有效地将大型DNA片段集成到基因功能研究中,大大推动了果遗传学的研究.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...