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Updated: Jul 1, 2026

FIBS-enabled Noninvasive Metabolic Profiling
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Published on: February 3, 2014

在培养中对原始生殖细胞存活率的巨细胞生长因子的要求

S Dolci1, D E Williams, M K Ernst

  • 1Mammalian Genetics Laboratory, NCl-Frederick Cancer Research and Development Center, Maryland 21702-1201.

Nature
|August 29, 1991
PubMed
概括

乳腺细胞生长因子 (MGF) 对于原始生殖细胞 (PGC) 的生存至关重要,但不是增殖. 跨膜MGF支持PGC的生存,解释了钢小鼠中的不育.

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media