相关实验视频
Updated: Jul 9, 2026

15:31
MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 12, 2010
收回:二次siRNAs来自未原始RNA合成,形成一个独特的类别
Titia Sijen1, Florian A Steiner, Karen L Thijssen
1Hubrecht Laboratory (NIOB-KNAW), Uppsalalaan 8, 3584 CT, the Netherlands.
概括
在C. elegans中,二次小干扰RNA (siRNA) 是由RNA指导的RNA聚合酶 (RdRP) 生产的. 这些siRNAs是不同的分子,由RdRP独立生成,并在RNA干扰 (RNAi) 中发挥关键作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 在C. elegans中,有效的RNA干扰 (RNAi) 取决于二级小干扰RNA (siRNA).
- 导向RNA的RNA聚合酶 (RdRPs) 对于产生这些二次siRNAs至关重要.
研究的目的:
- 描述C. elegans中二级siRNAs的生物发生和特性.
- 确定RdRPs.的二次siRNA产生机制.
主要方法:
- 从转基因C. elegans线路中克隆二次siRNAs.
- 分析二次siRNA序列及其与初级siRNA和目标mRNA的关系.
- 对二次siRNA极性,5'端修饰和与阿尔戈诺特蛋白质的关联进行了研究.
主要成果:
- 二级siRNAs起源于初级siRNAs的下游,表明非RISC分裂的mRNAs作为基质.
- RdRPs执行未开源的RNA合成,将补充核酸与mRNA结合起来,而不是不匹配.
- 二级siRNAs表现出反感极性,具有5'二或三酸盐,并且与阿尔戈纳特相关的siRNAs不同.
结论:
- 二级siRNAs代表了C. elegans中独特的一类小RNAs.
- 它们的生物发生是依赖RdRP的,每个二次siRNA可能是个别的RdRP产物.
- 了解二次siRNA生产,可以了解RNAi的放大机制.
相关概念视频
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

