Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electron Behavior00:54

Electron Behavior

Electrons are negatively charged subatomic particles that are attracted to an orbit around the positively-charged nucleus of an atom. They reside in locations that are associated with energy levels called shells and are further organized into sub-shells and orbitals within each shell.Electrons Orbit the NucleusElectrons are found in specific locations outside of the nucleus. The shell in which an electron resides indicates the general energy level of the electron: those closer to the nucleus...
Resting Membrane Potential01:24

Resting Membrane Potential

The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Induced Electric Dipoles01:28

Induced Electric Dipoles

A permanent electric dipole orients itself along an external electric field. This rotation can be quantified by defining the potential energy because the external torque does work in rotating it. Then, the potential energy is minimum at the parallel configuration and maximum at the antiparallel configuration. While the former is a stable equilibrium, the latter is an unstable equilibrium.
Since the absolute value of potential energy holds no physical meaning, its zero value can be chosen as per...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Electrochemical Systems01:24

Electrochemical Systems

Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution, the Zn metal, composed...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Simulating Metal-Imidazole Complexes.

Journal of chemical theory and computation·2024
Same author

Role of global public sector research in discovering new drugs and vaccines.

The Journal of technology transfer·2023
Same author

Protein oxidation involved in Cys-Tyr post-translational modification.

Journal of inorganic biochemistry·2017
Same author

Wide dynamic range sensing with single quantum dot biosensors.

ACS nano·2012
Same author

Identifying proteins that can form tyrosine-cysteine crosslinks.

Metallomics : integrated biometal science·2012
Same author

Submicron hard X-ray fluorescence imaging of synthetic elements.

Analytica chimica acta·2012

相关实验视频

Updated: Jul 8, 2026

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
12:20

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions

Published on: July 22, 2013

电子供体溶剂效应提供生物感知与量子点.

Baikuntha P Aryal1, David E Benson

  • 1Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.

Journal of the American Chemical Society
|December 15, 2006
PubMed
概括

一个新的palmitate生物传感器利用半导体纳米粒子和电子转移来检测palmitate度. 这种方法为开发各种小分子光成像试剂提供了一种新的方法.

科学领域:

  • 生物化学 生物化学
  • 纳米技术 纳米技术
  • 分析化学 分析化学

背景情况:

  • 生物传感器对于检测小分子至关重要.
  • 半导体纳米粒子为传感应用提供独特的光学特性.
  • 肠道脂肪酸结合蛋白 (IFABP) 在脂肪酸运输中起作用.

研究的目的:

  • 开发一种新的生物传感器,用于敏感的palmitate检测.
  • 为了利用半导体纳米粒子的光度来报告棕酸盐度.
  • 探索IFABP在调节生物传感器特性中的应用.

主要方法:

  • 使用涂有ZnS的CdSe纳米粒子构建了一个生物传感器.
  • 在纳米颗粒上共振地附加了一个修饰的复合物IFABP.
  • 利用纳米粒子发射的电子转移灭来检测palmitate.
  • 在添加棕酸盐后测量光强度的变化.

主要成果:

  • 观察到与500nM棕酸盐的排放强度的1.6倍变化.
  • 确定了palmitate的5nM的解离常数.
  • 确立了在1 nM的检测下限.

更多相关视频

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
04:53

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles

Published on: May 26, 2023

相关实验视频

Last Updated: Jul 8, 2026

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
12:20

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions

Published on: July 22, 2013

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
04:53

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles

Published on: May 26, 2023

  • 证明了棕酸盐结合会改变口袋溶解,而不会改变全球蛋白质构成.
  • 结论:

    • 开发了一种基于半导体纳米粒子的新型生物传感器.
    • 生物传感器具有很高的灵敏度和低的palmitate检测极限.
    • 这一策略可以扩展到检测数千种其他小分子分析剂.
    • 生物传感器可以作为光对比成像试剂.