Jove
Visualize
联系我们

相关概念视频

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A framework for reporting texture profile analysis in cultivated meat research.

Food chemistry·2026
Same author

Medical isotope-labeled nucleic acids: A decade of progress toward theranostics (2015-2025).

Current opinion in chemical biology·2026
Same author

Toward Multi(radio)metalated DNA: Enzymatic Polymerization of Metal-Chelate-Modified Deoxyribonucleoside Triphosphates.

Angewandte Chemie (International ed. in English)·2026
Same author

Total Synthesis of Orbiculamide A.

Organic letters·2026
Same author

A recipe for success: exploratory studies of the predictors of cultivated meat provision by parents of children 6-10 and 11-15 years of age.

Appetite·2026
Same author

In-Hospital Mortality Prediction of Patients Requiring Extracorporeal Membrane Oxygenation Using Composite Lactate Metrics and Weight-of-Evidence Modeling.

Journal of the American College of Surgeons·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 1, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

毛 ribozyme 中 G8 的活性位点标记:对结构和机制的影响.

Jason M Thomas1, David M Perrin

  • 1Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada V6T 1Z1.

Journal of the American Chemical Society
|December 21, 2006
PubMed
概括

研究人员在毛针 ribozyme 中确定了 guanosine 8 (G8) 作为催化过程中的关键成分. 这一发现,使用亲和度标签,表明G8可能充当一般基,类似于RNase A.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 酶学 是一种酶学.

背景情况:

  • 一般的酸催化被提议用于毛式 рибо酶,类似于RNase A.
  • 讨论了 ribozymes 中的特定催化组和一般基质催化作用的程度.

研究的目的:

  • 为了识别发针里波酶中负责一般基催化的化学群体.
  • 应用亲和性标记,一种用于RNase A的技术,对 ribozymes.

主要方法:

  • 合成了一种带有2'-乙胺组的亲和度标签.
  • 在基质裂解部位的发针 ribozyme 上涂上亲和度标签.

主要成果:

  • 特殊的基化瓜诺辛8 (G8) 的毛 ribozyme,可能在N1位置.
  • 这种化涉及G8的N1在利博酶的活性部位化学中.

结论:

  • 这些发现支持了这样一个假设:G8的N1在毛 ribozyme 催化过程中起到一般的基因作用,模仿RNase A.
  • 其他机制,包括水媒介催化或G8的过渡状态稳定,也是可信的.

更多相关视频

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

相关实验视频

Last Updated: Jul 1, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022