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Updated: Jul 8, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

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通过基于电子束的无活化对细胞外矩阵蛋白的高保真性功能模式

Jonas Rundqvist1, Beatriz Mendoza, Jeffrey L Werbin

  • 1Nanostructure Physics, Royal Institute of Technology, AlbaNova University Center, Roslagsvägen 30B, SE-106 91 Stockholm, Sweden.

Journal of the American Chemical Society
|January 4, 2007
PubMed
概括

电子束写作精确地图案纤维菌素表面,通过在纳米尺度上禁用蛋白质来控制细胞行为. 这种高保真度的方法为研究细胞信号和空间组织提供了新的工具.

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.